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INH6

Cat. No. M5282
INH6 Structure
Size Price Availability Quantity
10mg USD 80 In stock
50mg USD 300 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

INH6 effectively targets the Hec1/Nek2 complex for degradation, and shows potent cell killing activity with IC50 of 1.7, 2.1, 2.4, and 2.5 μM in MDA-MB231, MDA-MB468, HeLa and K562 cell lines. INH6 also triggers mitotic abnormalities in HeLa cells, and induces apoptosis.

Protocol
Cell Experiment
Cell lines MDA-MB231, MDA-MB468, HeLa and K562 cell lines
Preparation method Standard XTT assays with a four-day drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH analogs in cultured cells. Triplicate sets are measured and compiled for final data presentation. The assay is performed by using a commercial kit following the instructions. In principle, cells are plated on 96-well dishes one day before the drug treatment, followed by drug treatment on day 2 and XTT assay on day 5 after drug addition. The absorption at 595 nm is measured with a plate reader and converted to cell survival percentages in comparison to mock treated groups.
Concentrations ~40 μM
Incubation time 4 days
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 322.42
Formula C19H18N2OS
CAS Number 1001753-24-7
Purity >99%
Solubility 64 mg/mL in DMSO
Storage at -20°C
References

Inhibition of the peroxidase-catalyzed oxidation of chromogenic substrates by alkyl-substituted diatomic phenols
Naumchik IV, et al. Bioorg Khim. 2004 Sep;30(5):537-46. PMID: 15562976.

Enhancement of the performance of gas hydrate kinetic inhibitors with polyethylene oxide
JD Lee, et al. Chemical Engineering Science 2005 ; 5323 – 5330.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: INH6 supplier, Microtubule, inhibitors

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