Dihydroethidium (also known as Hydroethidine and PD-MY 003) is a cell-permeable blue fluorescent dye. DHE-derived fluorescence at 570 nm is a convenient method for detection of intracellular and extracellular superoxide produced by phagocytic and vascular NADPH oxidase. Dihydroethidium is neuroprotective by reducing superoxide in mice after stroke.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO ≥ 45 mg/mL|
|Storage||2-8°C, protect from light, dry, sealed|
On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment
Radoslaw Michalski, et al. Free Radic Biol Med. 2014 Feb;67:278-84. PMID: 24200598.
Global profiling of reactive oxygen and nitrogen species in biological systems: high-throughput real-time analyses
Jacek Zielonka, et al. J Biol Chem. 2012 Jan 27;287(5):2984-95. PMID: 22139901.
Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products
Francisco R M Laurindo, et al. Methods Enzymol. 2008;441:237-60. PMID: 18554538.
Generation of superoxide anion by equine spermatozoa as detected by dihydroethidium
L Burnaugh, et al. Theriogenology. 2007 Feb;67(3):580-9. PMID: 17045638.
Treatment with dihydroethidium reduces infarct size after transient focal cerebral ischemia in mice
Fengshan Yu, et al. Brain Res. 2003 Jul 18;978(1-2):223-7. PMID: 12834917.
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