HU (100 μm), significantly increased the phosphorylation of 3TC in HepG2 cells, with significant reductions in dCTP also noted at this concentration. The resulting 3TCTP/dCTP ratio was increased dramatically. The mechanism behind this interaction involves the production of dCTP itself. dCTP is produced from two sources, the reduction of circulating cytidine-DP (CDP) to form dCDP, which is then phosphorylated to dCTP (the de novo pathway), and, the sequential phosphorylation of dC to dCTP (the dC salvage pathway).
|Cell lines||HepG2 cell|
|Preparation method||Extracts were stored at −20°C until determination of dCTP pools by a similar procedure to that described previously. Reaction mixtures (100 μl) contained 0.2μCi [3H]-dATP, 0.25 μm synthetic oligonucleotide primer, 10 mm MgCl2, 5 mm dithiothreitol, 50 mm Tris-HCl (pH 7.5) and 1 unit Sequenase 2.0 enzyme.|
|Incubation time||24 h|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||10mM in DMSO|
Capacity of N4-methyl-2'-deoxycytidine 5'-triphosphate to sustain the polymerase chain reaction using various thermostable DNA polymerases.
Flores-Juárez CR, et al. Anal Biochem. 2013 Jul 1;438(1):73-81. PMID: 23548504.
The intracellular activation of lamivudine (3TC) and determination of 2′-deoxycytidine-5′-triphosphate (dCTP) pools in the presence and absence of various drugs in HepG2 cells
Stephen Kewn, et al. Br J Clin Pharmacol. 2000 Dec;50(6): 597–604. PMID: 11136299.
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