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Deoxycytidine triphosphate

Cat. No. M5300
Deoxycytidine triphosphate Structure
Synonym:

dCTP

Size Price Availability
10mg USD 100 1-2 Weeks
50mg USD 225 1-2 Weeks
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

HU (100 μm), significantly increased the phosphorylation of 3TC in HepG2 cells, with significant reductions in dCTP also noted at this concentration. The resulting 3TCTP/dCTP ratio was increased dramatically. The mechanism behind this interaction involves the production of dCTP itself. dCTP is produced from two sources, the reduction of circulating cytidine-DP (CDP) to form dCDP, which is then phosphorylated to dCTP (the de novo pathway), and, the sequential phosphorylation of dC to dCTP (the dC salvage pathway).

Protocol
Cell Experiment
Cell lines HepG2 cell
Preparation method Extracts were stored at −20°C until determination of dCTP pools by a similar procedure to that described previously. Reaction mixtures (100 μl) contained 0.2μCi [3H]-dATP, 0.25 μm synthetic oligonucleotide primer, 10 mm MgCl2, 5 mm dithiothreitol, 50 mm Tris-HCl (pH 7.5) and 1 unit Sequenase 2.0 enzyme.
Concentrations --
Incubation time 24 h
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 467.16
Formula C9H16N3O13P3
CAS Number 2056-98-6
Purity >99%
Solubility 10mM in DMSO
Storage at -20°C
References

Capacity of N4-methyl-2'-deoxycytidine 5'-triphosphate to sustain the polymerase chain reaction using various thermostable DNA polymerases.
Flores-Juárez CR, et al. Anal Biochem. 2013 Jul 1;438(1):73-81. PMID: 23548504.

The intracellular activation of lamivudine (3TC) and determination of 2′-deoxycytidine-5′-triphosphate (dCTP) pools in the presence and absence of various drugs in HepG2 cells
Stephen Kewn, et al. Br J Clin Pharmacol. 2000 Dec;50(6): 597–604. PMID: 11136299.

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Keywords: Deoxycytidine triphosphate, dCTP supplier, DNA/RNA Synthesis, inhibitors

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