Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, inhibits the enlongation and shortening of actin filaments, with Kds of 2.2 nM and 1.4 nM for F-actin in the presence of MgCl2 (2 mM) or MgCl2 (2 mM) plus KCl, respectively. Cytochalasin B (6 μM) increases the myofibrillar fragmentation index (MFI), which is attributed to the intensely breaking of myofibrillar proteins into short segments. Cytochalasin B also accelerates the disruption of actin filaments.
In vivo, Cytochalasin B (10, 25, 50 mg/kg, i.p.) dose-dependently increases the life expectancy of Balb/c mice bearing with P388/ADR leukemias. Cytochalasin B at 50 mg/kg produces 10 % long-term survival in the multidrug resistant P388/ADR cohort, and 40 % long-term survival in the drug sensitive P388/S cohort.
Cell Commun Signal. 2023 Jan 23;21(1):22.
Extracellular matrix stiffness mediates uterine repair via the Rap1a/ARHGAP35/RhoA/F-actin/YAP axis
Cytochalasin B purchased from AbMole
Cells. 2022 Oct 9;11(19):3162.
Crosstalk between Extracellular Matrix Stiffness and ROS Drives Endometrial Repair via the HIF-1α/YAP Axis during Menstruation
Cytochalasin B purchased from AbMole
| Molecular Weight | 479.61 |
| Formula | C29H37NO5 |
| CAS Number | 14930-96-2 |
| Form | Solid |
| Solubility (25°C) | DMSO 10 mM |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Actin-Related Protein Products |
|---|
| EG-011
EG-011 is a first-in-class, potent Wiskott-Aldrich Syndrome Protein (WASP) activator that activates the self-inhibited form of WASP via strong actin polymerization.In addition, EG-011 has selective anti-lymphoma activity. |
| Dihydrocytochalasin B
Dihydrocytochalasin B is a Cytokinesis inhibitor and changes the morphology of the cells, similar to that of cytochalasin B. |
| Jasplakinolide
Jasplakinolide is an actin-specific reagent that promotes actin polymerization and stabilizes actin filaments. |
| Cytochalasin D
Cytochalasin D is a cytopermeable actin polymerization inhibitor derived from fungi, which inhibits g-actin -- Cofilin interaction by binding g-actin. Cytochalasin D can also inhibit the binding of Cofilin to F-actin, reduce the release of exosomes and induce YAP phosphorylation. |
| SMIFH2
SMIFH2 is a fH2 domain inhibitor; prevents actin nucleation. |
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