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Cyclophosphamide

Cat. No. M3746
Cyclophosphamide Structure
Size Price Availability Quantity
50mg USD 60 In stock
500mg USD 280 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Cyclophosphamide inhibits the AChE reversibly with an IC50 of 511 microM. The Michaelis-Menten constant (Km) was 132 microM for AChE in the control system; a value increased by 78% in the CP treated system. The Vmax was 73.8 mumol/h/mg protein for the control system. Cyclophosphamide is used to treat cancers and autoimmune disorders. Cyclophosphamide belongs to a group of drugs called alkylating agents. It works by sticking to one of the cancer cell's DNA strands. DNA is the genetic code that is in the heart of all animal and plant cells. It controls everything the cell does.The cell cannot then divide into 2 new cells.

Protocol
Cell Experiment
Cell lines CD4+ T-cell
Preparation method Total CD4+ T cells, labeled with αCD25 BV605, were cultured for 3 days in RPMI/10% AB containing 0 or 50 IU/mL IL-2 as indicated, in presence of CPA (0 μM-1000 μM), mafosfamide (MAF; stabilized active derivative of CPA) (0 μM, 1.2 μM, 12 μM), and/or verapamil (10 μM). Doses of CPA and MAF were titrated to identify doses discriminating sensitivity of Treg and non-Treg cell subsets.
Concentrations 0 μM, 1.2 μM, 12 μM
Incubation time 3 d
Animal Experiment
Animal models Sprague–Dawley rats
Formulation
Dosages 25mg/kg
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 261.09
Formula C7H15Cl2N2O2P
CAS Number 50-18-0
Purity >99%
Solubility DMSO 52mg/mL
Water 43 mg/mL
Ethanol 52 mg/mL
Storage at -20°C
Customer Product Validations & Biological Datas
Source Mol Cancer Ther (2015). Figure 5.Cyclophosphamide
Method i.p.
Cell Lines NCR/nu and nu/nu nude mice
Concentrations 170 mg/kg
Incubation Time 24 h
Results Cyclophosphamide induced a significant increase of DiI5-liposome MFI at each of the distances from blood vessels analyzed.
Rating
Source Mol Cancer Ther (2015). Figure 1.Cyclophosphamide
Method i.p.
Cell Lines NCR/nu and nu/nu nude mice
Concentrations 170 mg/kg
Incubation Time 24 h
Results BT474-M3 tumor–bearing mice were predosed with cyclophosphamide (170 mg/kg) 2 to 5 days before injection of HER2-tPLD or free doxorubicin (both at 3 mg/kg).
Rating
References

Human regulatory T cells lack the cyclophosphamide-extruding transporter ABCB1 and are more susceptible to cyclophosphamide-induced apoptosis.
Dimeloe S, et al. Eur J Immunol. 2014 Dec;44(12):3614-20. PMID: 25251877.

Inhibition of human acetylcholinesterase by cyclophosphamide.
al-Jafari AA, et al. Toxicology. 1995 Jan 19;96(1):1-6. PMID: 7863507.

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Keywords: Cyclophosphamide supplier, DNA/RNA Synthesis, inhibitors

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