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Cucurbitacin-E

Cat. No. M4617
Cucurbitacin-E Structure
Synonym:

α-Elaterin; α-Elaterine

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20mg USD 360  USD360 4-7 Days
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Quality Control & Documentation
Biological Activity

Cucurbitacin-E is a natural compound which from the climbing stem of Cucumic melo L, which significantly suppresses the activity of the cyclin B1/CDC2 complex.

Protocol (for reference only)
Cell Experiment
Cell lines The colorectal cancer (CRC) cells
Preparation method The colorectal cancer (CRC) cells are seeded into 96-well culture plates at 5000 cells/well. The cells are treated with 0, 2.5, 5, and 7.5 μM Cucurbitacin E for 1-3 days. MTT dye (1 mg/mL) is added to each well for at least 4 h of treatment. The reaction is stopped by the addition of DMSO, and optical density is measured at 540 nm on a multi-well plate reader. Background absorbance of the medium in the absence of cells is subtracted. All samples are assayed in triplicate, and the mean for each experiment is calculated. Results are expressed as a percentage of control, which is considered as 100%. Each assay is carried out in triplicate, and the results are expressed as the mean.
Concentrations 0, 2.5, 5, and 7.5 μM
Incubation time 1-3 days
Animal Experiment
Animal models C57BL/6 male mice
Formulation
Dosages 0.25, 0.5 mg/kg/day
Administration oral gavage
Chemical Information
Molecular Weight 556.69
Formula C32H44O8
CAS Number 18444-66-1
Solubility (25°C) DMSO 10 mM
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Murtaza M, et al. PLoS One. Cucurbitacin E reduces obesity and related metabolic dysfunction in mice by targeting JAK-STAT5 signaling pathway.

[2] Hsu YC, et al. Cell Death Dis. Therapeutic ROS targeting of GADD45γ in the induction of G2/M arrest in primary human colorectal cancer cell lines by cucurbitacin E.

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Keywords: Cucurbitacin-E, α-Elaterin; α-Elaterine supplier, CDK, inhibitors, activators


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