Collagenase (Type II) is a protease derived from the fermentation of Clostridium histolyticus.
Collagenase: 0.2% (w/v) collagenase solution made up in Hank’s balanced salt solution (HBSS); filter sterilise.
Note that HBSS contains calcium, which is required for collagenase activity.
| Molecular Weight | 849.45 |
| Formula | C60H100N2 |
| CAS Number | 9001-12-1 |
| Solubility (25°C) | Soluble in aqueous buffers |
| Storage | -20°C, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Isabel R Orriss, et al. Methods Mol Biol. Rat osteoblast cultures
| Related Enzymes & Coenzymes Products |
|---|
| Aeromonas proteolytica aminopeptidase
Aeromonas proteolytica aminopeptidase is a biochemical reagent that can be used as a biological material or organic compound for life science related research. |
| Pyroglutamate aminopeptidase I
Pyroglutamate aminopeptidase I is a biochemical reagent that can be used as a biological material or organic compound for life science related research. |
| Matrix metalloproteinase 3
Matrix metalloproteinase 3 is a biochemical reagent that can be used as a biological material or organic compound for life science related research. |
| γ-Glutamyl hydrolase
γ-Glutamyl hydrolase is a biochemical reagent that can be used as a biological material or organic compound for life science related research. |
| Cathepsin C
Cathepsin C is a biochemical reagent that can be used as a biological material or organic compound for life science related research. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.
