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Colchicine

Cat. No. M3460
Colchicine Structure
Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM*1mL In DMSO USD 70 In stock
100mg USD 50 In stock
500mg USD 80 In stock
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Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM. Colchicine is a toxic natural product and secondary metabolite, originally extracted from plants of the genus Colchicum. Colchicine inhibited stimulated endothelial adhesiveness via its effects on microtubules since vinblastine, an agent which perturbs microtubule function by other mechanisms, diminished adhesiveness whereas the photoinactivated colchicine derivative gamma-lumicolchicine was inactive. Colchicine (Colchineos, Colchisol, Colcin) had no effect on cell viability. At higher, therapeutic, concentrations colchicine (IC50 = 300 nM, P < 0.001) also diminished the expression of L-selectin on the surface of neutrophils (but not lymphocytes) without affecting expression of the beta 2-integrin CD11b/CD18. Since one of the defining characteristics of cancer cells is a significantly increased rate of mitosis, this means that cancer cells are significantly more vulnerable to colchicine poisoning than are normal cells. However, the therapeutic value of colchicine against cancer is (as is typical with chemotherapy agents) limited by its toxicity against normal cells.

Customer Product Validations & Biological Datas
Source Cancer Res (2017). Figure 2. Colchicine
Method Scratch migration assay
Cell Lines A375 and RPMI7951 cells
Concentrations 25 nM
Incubation Time 12 and 24 h
Results The effect of DJ101 on A375 cell colony formation was similar to colchicine at the same concentration, which had colonies covering a total area of 34.7% ± 1.5%
Protocol
Cell Experiment
Cell lines COS-7 cells
Preparation method After 20 min, the samples were mixed with 5 nM CT20126, colchicine or Taxol and incubated at 37 °C for 5 min. The samples were cooled in an ice bath for depolymerization (still within the light path cell). Successive reactions were performed for another 25 min at 37 °C.
Concentrations 5 nM
Incubation time 5 min
Animal Experiment
Animal models Male Sprague–Dawley rats
Formulation water
Dosages 5 mg/kg
Administration gavage
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 399.44
Formula C22H25NO6
CAS Number 64-86-8
Purity >98%
Solubility DMSO: ≥ 45 mg/mL
Storage at -20°C
References

Fab fragments of ovine antibody to colchicine enhance its clearance in the rat.
Peake PW, et al. Clin Toxicol (Phila). 2015 Jun;53(5):427-32. PMID: 25858137.

The colchicine derivative CT20126 shows a novel microtubule-modulating activity with apoptosis.
Kim SK, et al. Exp Mol Med. 2013 Apr 19;45:e19. PMID: 23598593.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Colchicine supplier, Microtubule, inhibitors

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