Coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 μM/ml) shows a bioluminescence signal.
In vivo, in 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO: ≥ 40 mg/mL|
|Storage||-20°C, protect from light|
Multimodality Imaging of Cancer Superoxide Anion Using the Small Molecule Coelenterazine.
Bronsart LL, et al. Mol Imaging Biol. 2016 Apr;18(2):166-71. PMID: 26420297.
A water-soluble coelenterazine for sensitive in vivo imaging of coelenterate luciferases.
Morse D, et al. Mol Ther. 2012 Apr;20(4):692-3. PMID: 22472977.
Imaging reversal of multidrug resistance in living mice with bioluminescence: MDR1 P-glycoprotein transports coelenterazine.
Pichler A, et al. Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1702-7. PMID: 14755051.
Preladenant is a potent and competitive antagonist of the human adenosine A2A receptor with a Ki of 1.1 nM.
|Recombinant Human GM-CSF (CHO-expressed)
Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor (CHO-expressed) is a hematopoietic growth factor and immune modulator.
Glutathione oxidized is produced by the oxidation of glutathione.
NG25 is a potent dual TAK1 and MAP4K2 inhibitor with IC50s of 149 nM and 21.7 nM, respectively.
|IRAK-1-4 Inhibitor I
IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC50s of 0.3 μM and 0.2 μM, respectively.
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