Cholic acid is a major primary bile acid produced in the liver and usually conjugated with glycine or taurine. It facilitates fat absorption and cholesterol excretion.
| Cell Experiment | |
|---|---|
| Cell lines | HepG2 cells |
| Preparation method | HepG2 cells are pretreated with the inhibitors NaN3 (1 mg/mL), Genistein (50 μg/mL), MβCD (10 mM), Nystatin (50 μg/mL), Chlorpromazine (10 μg/mL), and Cholic acid (1 mg/mL) for 30 min. After removing the inhibitors, the cells are incubated with CA-LPs for 2 h, and the cellular uptake of LPs is determined in the "In vitro cellular uptake assays" section. |
| Concentrations | 1 mg/mL |
| Incubation time | 30 min |
| Animal Experiment | |
|---|---|
| Animal models | Tg-CYP2D6 mice |
| Formulation | 1% (w/w) Cholic acid-supplemented |
| Dosages | 14 days |
| Administration | fed |
| Molecular Weight | 408.57 |
| Formula | C24H40O5 |
| CAS Number | 81-25-4 |
| Solubility (25°C) | DMSO: ≥ 40 mg/mL |
| Storage | 4°C, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Metabolite/Endogenous Metabolite Products |
|---|
| D-Thyroxine
D-Thyroxine is a thyroid hormone that can inhibit TSH secretion. D-Thyroxine can be used for the research of hypercholesterolemia. |
| Allolithocholic acid
Allolithocholic acid is a steroid acid could found in normal serum and feces. Allolithocholic acid facilitates excretion, absorption, and transport of fats and sterols in the intestine and liver. |
| Biliverdin
Biliverdin is the product of the oxidation of bilirubin. Biliverdin can be synthesized via a non-protoporphyrin pathway in Corynebacterium glutamicum. |
| Stachyose hydrate
Stachyose tetrahydrate is a functional oligosaccharide, acts as a prebiotic. Stachyose tetrahydrate can prevent indirectly colon cancer cell growth by promoting the proliferation of probiotics or producing beneficial materials in the intestine. Stachyose hydrate exhibit a hypoglycemic effect, and improve inflammation through modulating gut microbiota. |
| Stachyose tetrahydrate
Stachyose tetrahydrate is a functional oligosaccharide, acts as a prebiotic. Stachyose tetrahydrate can prevent indirectly colon cancer cell growth by promoting the proliferation of probiotics or producing beneficial materials in the intestine. |
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