Carboplatin is a second-generation platinum compound with a broad spectrum of antineoplastic properties. Carboplatin is activated intracellularly to form reactive platinum complexes that bind to nucleophilic groups such as GC-rich sites in DNA, thereby inducing intrastrand and interstrand DNA cross-links, as well as DNA-protein cross-links. Carboplatin possesses tumoricidal activity similar to that of its parent compound, cisplatin, but is more stable and less toxic. Carboplatin is used against some forms of cancer (mainly ovarian carcinoma, lung, head and neck cancers).
|Cell lines||5637 cells|
|Preparation method||Cells were dosed and incubated with 290 μM carboplatin or 290 μM carboplatin combined with 0.08 μM paclitaxel for 4 h, each including 0.43 μM[14C]carboplatin (106 dpm/ml). After washing, cells were maintained with 14C carboplatin-free culture media. DNA was extracted from cells and converted to graphite and measured by AMS for 14C quantification as previously described.|
|Incubation time||4 h|
|Animal models||Female 6‐week‐old nude mice|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Source||Mol Oncol (2014). Figure 4. Carboplatin|
|Cell Lines||Female 6-week-old nude mice|
|Incubation Time||5 weeks|
|Results||Both carboplatin treatment groups showed significantly reduced tumor burden as compared to the untreated controls from weeks 4 to 10 (P < 0.0001)|
Paclitaxel Enhances Carboplatin-DNA Adduct Formation and Cytotoxicity.
Jiang S, et al. Chem Res Toxicol. 2015 Dec 21;28(12):2250-2. PMID: 26544157.
Enhancement of paclitaxel and carboplatin therapies by CCL2 blockade in ovarian cancers.
Moisan F, et al. Mol Oncol. 2014 Oct;8(7):1231-9. PMID: 24816187.
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