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Calcein, AM

Cat. No. M5114
Calcein, AM Structure

Calcein acetoxymethyl ester

Size Price Availability Quantity
50ug USD 75 In stock
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Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability. The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 994.86
Formula C46H46N2O23
CAS Number 148504-34-1
Purity >98%
Solubility DMSO ≥ 6 mg/mL
Storage -20°C, protect from light, dry, sealed

Novel Fluorescence Assay Using calcein-AM for the Determination of Human Erythrocyte Viability and Aging
Daniela Bratosin, et al. Cytometry A. 2005 Jul;66(1):78-84. PMID: 15915509.

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Keywords: Calcein, AM, Calcein acetoxymethyl ester supplier, Fluorescent Dye, inhibitors

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