Free shipping on all orders over $ 500
Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability. The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane.
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
| Molecular Weight | 994.86 |
| Formula | C46H46N2O23 |
| CAS Number | 148504-34-1 |
| Purity | >98% |
| Solubility | DMSO ≥ 6 mg/mL |
| Storage | -20°C, protect from light, dry, sealed |
Novel Fluorescence Assay Using calcein-AM for the Determination of Human Erythrocyte Viability and Aging
Daniela Bratosin, et al. Cytometry A. 2005 Jul;66(1):78-84. PMID: 15915509.
| Related Fluorescent Dye Products |
|---|
| Fluo 3-AM |
| Streptavidin-FITC
Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. |
| Coumarin 6
Coumarin 6 is a fluorescent dye frequently used to facilitate the traceability of drug delivery systems in vitro. |
| Calcein
Calcein is a fluorescent dye and self-quenching probe, used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. |
| 4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene
4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene (BODIPY 493/503) is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.
