Free shipping on all orders over $ 500

Calcein, AM

Cat. No. M5114
Calcein, AM Structure
Synonym:

Calcein acetoxymethyl ester

Size Price Availability Quantity
50ug USD 75 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability. The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 994.86
Formula C46H46N2O23
CAS Number 148504-34-1
Purity >98%
Solubility DMSO ≥ 6 mg/mL
Storage -20°C, protect from light, dry, sealed
References

Novel Fluorescence Assay Using calcein-AM for the Determination of Human Erythrocyte Viability and Aging
Daniela Bratosin, et al. Cytometry A. 2005 Jul;66(1):78-84. PMID: 15915509.

Related Fluorescent Dye Products
Fluo 3-AM

Streptavidin-FITC

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l.

Coumarin 6

Coumarin 6 is a fluorescent dye frequently used to facilitate the traceability of drug delivery systems in vitro.

Calcein

Calcein is a fluorescent dye and self-quenching probe, used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene

4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene (BODIPY 493/503) is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Calcein, AM, Calcein acetoxymethyl ester supplier, Fluorescent Dye, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.