Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability. The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane.
Chemical Engineering Journal. 2023 Dec 15;147407.
Pitaya-inspired microcarrier/hydrogel composite for chronic wound healing: One-pot preparation, tailorable structures, and versatile functionalization
Calcein-AM purchased from AbMole
Applied Materials Today. 2023 May 14.
Hierarchically hollow microcapsules with antibacterial and angiogenic properties for abdominal wall defects treatment
Calcein-AM purchased from AbMole
2023 Jun.
LncRNA GHET1 from bone mesenchymal stem cell–derived exosomes improves doxorubicin-induced pyroptosis of cardiomyocytes by mediating NLRP3
Calcein-AM purchased from AbMole
AMACS Appl Mater Interfaces. 2022 Feb 2;14(4):5033-5052.
Trojan-Horse Diameter-Reducible Nanotheranostics for Macroscopic/Microscopic Imaging-Monitored Chemo-Antiangiogenic Therapy
Calcein-AM purchased from AbMole
new journal of chemistry. 2022 Nov 24;47, 628.
Carbon-based dot-capped gold nanoparticles hybridized with manganese dioxide for enhanced photodynamic cancer therapy
Calcein-AM purchased from AbMole
Nat Commun. 2020 Dec 22;11(1):6438.
MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression
Calcein-AM purchased from AbMole
| Molecular Weight | 994.86 |
| Formula | C46H46N2O23 |
| CAS Number | 148504-34-1 |
| Solubility (25°C) | DMSO ≥ 6 mg/mL |
| Storage | -20°C, protect from light, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Fluorescent Dye Products |
|---|
| SOSG
SOSG (Singlet Oxygen Sensor Green Fluorescent Probe) is highly selective for singlet oxygen (1O2). It is commonly used for the detection of singlet oxygen in solutions and the evaluation of the efficacy of free radical scavengers. |
| FM1-43
FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers. |
| BCIP
BCIP is a chromogenic substrate for colorimetric assay of alkaline phosphatase activity. |
| MCA-SEVNLDAEFR-K(Dnp)-RR, amide
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate. |
| MeOSuc-AAPV-AMC
MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm). |
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