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BV6

Cat. No. M3638
BV6 Structure
Size Price Availability Quantity
2mg USD 100 In stock
5mg USD 145 In stock
10mg USD 250 In stock
50mg USD 815 In stock
100mg USD 1020 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

BV6 induces autoubiquitination and subsequent proteasomal degradation of cIAP1 and cIAP2, and greatly sensitizes cells to TNF-induced necrosis. BV6 significantly enhances the radiosensitization of HCC193 and H460 cells in vitro. BV6 sensitizes both cell lines to radiation (HCC193-DER = 1.38, p < 0.05 at 1 μM BV6; H460-DER = 1.42, p < 0.05 at 5 μM BV6), but a higher concentration of and longer incubation time with BV6 was necessary for H460 cells.

Protocol
Cell Experiment
Cell lines HCC193 and H460 cells
Preparation method Using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay kit to measure cell viability . Seeding 5000 cells/well into 96-well plates in triplicate. Following adhesion of cells to the wells, adding increasing concentrations of BV6 into different wells.Exposing control groups to the same concentration of DMSO. Addng the final concentrations of 333 μg/mL MTS and 25 μM PMS to each well 24 hours later. After two hours incubation at 37 °C in humidified 5% CO2, read plates at the absorbance of 490-nm on a microplate reader.Calculating relative cell viability of an individual sample by normalizing their absorbance to that of the corresponding control. Using Prism 5.01 to calculate IC50 values . For the TNFα neutralizing antibody assay, exposing cells to 1 and 5 μM BV6 with or without 10 μg/mL infliximab and the assay is performed 24 hours later. Read plates at the absorbance of 490-nm on a microplate reader
Concentrations ~30 μM
Incubation time ~48 hours
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 1205.57
Formula C70H96N10O8
CAS Number 1001600-56-1
Purity >99%
Solubility DMSO
Storage at -20°C
References

BV6, an IAP antagonist, activates apoptosis and enhances radiosensitization of non-small cell lung carcinoma in vitro.
Li W, et al. J Thorac Oncol. 2011 Nov;6(11):1801-9. PMID: 21760551.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: BV6 supplier, IAP, inhibitors

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