Recombinant Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). BMP-2 plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.
Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells, the ED50 for this effect is typically 0.07-0.2 µg/ml.
Measured by its ability to induce alkaline phosphatase production by C2C12 cells, the ED50 for this effect is typically 0.2-1 µg/ml.
Accession: # P12643
Apparent Molecular Weight: ~26 kDa, on SDS-PAGE under non-reducing conditions.
Lyophilized after extensive dialysis against 50 mM acetic acid.
Solubility (25°C) | Reconstitute the lyophilized powder in 20 mM AcOH or 5 mM HCl up to 100 μg/ml. |
Storage | Stable for up to 6 months at lower than -70°C |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Branko Skovrlj, et al. Spine (Phila Pa 1976). Association Between BMP-2 and Carcinogenicity
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