Berberrubine
 |
Source | Asian Pac J Cancer Prev (2015). Figure 4. Berberrubine |
| Method | DPPH assay | |
| Cell Lines | HT1080 cells | |
| Concentrations | 0.1 and 1.0 μM | |
| Incubation Time | 7 d | |
| Results | In contrast, B2-B4 did not show the significant difference in CAT expression if compared with B1. Interestingly, compounds B1-B4 exhibited a significant up-regulation of SOD expression in a dose dependent fashion (p<0.05) compared with the untreated cells. |
 |
Source | Asian Pac J Cancer Prev (2015). Figure 3. Berberrubine |
| Method | MTT assay | |
| Cell Lines | HT1080 cells | |
| Concentrations | 0.1 and 1.0 μM | |
| Incubation Time | 7 d | |
| Results | The result of cytotoxicity (Figure 3) showed that compounds B1, B2, and B4 significantly decreased the viability of HT1080 at all concentrations with both dose and time-dependent fashion (p<0.05). |
| Cell Experiment | |
|---|---|
| Cell lines | AMC5 cells |
| Preparation method | AMC5/B1 cells were selected by intermittent exposure of surviving cells to increasing concentrations of berberrubine. AMC5 cells in 100-mm plastic dishes were first exposed to berberrubine at 10 μM to reduce the surviving fraction to 20 to 30% and were allowed to grow in the absence of selecting drug until they reached the initial cell density or greater. |
| Concentrations | 10 μM |
| Incubation time | 16 h |
| Animal Experiment | |
|---|---|
| Animal models | C57BL/6J mice |
| Formulation | |
| Dosages | 25, 50 mg/kg |
| Administration | p.o. |
| Molecular Weight | 357.79 |
| Formula | C19H16ClNO4 |
| CAS Number | 15401-69-1 |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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