AEBSF HCl is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin. AEBSF hydrochloride inhibits the constitutive production of A beta in five different human cell lines, both neural and nonneural. AEBSF HCl also stabilizes full-length beta APP and enhances alpha-secretion, as shown by an increase in the proteolytic derivative, alpha-APPS. AEBSF (76.8 mg/kg daily i.p.) results in prolongation of the survival of mice that have been given a lethal T. gondii infection. AEBSF also reduces airway response and underlying inflammation in cockroach allergen-induced murine model.
Front Immunol. 2023 Mar 15;14:1127552.
Giardia duodenalis-induced G0/G1 intestinal epithelial cell cycle arrest and apoptosis involve activation of endoplasmic reticulum stress in vitro
AEBSF HCl purchased from AbMole
Cell Experiment | |
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Cell lines | HUVECs |
Preparation method | When HUVECs were grown to 60%∼70% confluence, the culture medium was changed, and 150 μL new media with different concentrations of AEBSF (0, 10, 20 mcg/mL) were added to the adherent cells. After 24 h of the AEBSF treatment, 50 μL BCECF-AM- labeled HeLa cells (ATCC) were seeded onto HUVEC monolayers (105 cells/well) and co-cultured for 4 h. |
Concentrations | 0, 10, 20 mcg/mL |
Incubation time | 24 h |
Animal Experiment | |
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Animal models | Adult Sprague–Dawley (SD) rats |
Formulation | saline |
Dosages | 5 or 10 mg/kg |
Administration | i.e. |
Molecular Weight | 239.69 |
Formula | C8H11ClFNO2S |
CAS Number | 30827-99-7 |
Solubility (25°C) | Water ≥ 90 mg/mL DMSO ≥ 60 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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