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5-methoxyflavone

Cat. No. M7506
5-methoxyflavone Structure
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Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

The treatment with 5-methoxyflavone (5-MF) at various times (3, 6, 12, 18 and 24 h) in MOLT-4 cells results in a time dependent reduction in the levels of antiapoptotic proteins cFLIP, Mcl-1 and an increase in the proapoptotic protein BAX. 5-MF induces both TRAIL-R1(DR4) and TRAIL-R2 (DR5) in a time-dependent manner

Protocol
Cell Experiment
Cell lines human leukemic MOLT-4, U937 cell lines and PBMCs
Preparation method MOLT-4 or U937 or PBMC cells are treated with DMF or TMF or PMF, 5-MF or 2'-MF at various concentrations for 24 h, then the cell viability is determined by the MTT assay.
Concentrations 0-100 μg/ml
Incubation time 24 h
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 252.26
Formula C16H12O3
CAS Number 42079-78-7
Purity >98%
Solubility 10 mM in DMSO
Storage at -20°C
References

Identification of 5-Methoxyflavone as a Novel DNA Polymerase-Beta Inhibitor and Neuroprotective Agent against Beta-Amyloid Toxicity.
Merlo S, et al. J Nat Prod. 2015 Nov 25;78(11):2704-11. PMID: 26517378.

Methoxyflavone derivatives modulate the effect of TRAIL-induced apoptosis in human leukemic cell lines.
Wudtiwai B, et al. J Hematol Oncol. 2011 Dec 21;4:52. PMID: 22185222.

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  Catalog
Abmole Inhibitor Catalog 2017




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