The treatment with 5-methoxyflavone (5-MF） at various times (3, 6, 12, 18 and 24 h) in MOLT-4 cells results in a time dependent reduction in the levels of antiapoptotic proteins cFLIP, Mcl-1 and an increase in the proapoptotic protein BAX. 5-MF induces both TRAIL-R1(DR4) and TRAIL-R2 (DR5) in a time-dependent manner
|Cell lines||human leukemic MOLT-4, U937 cell lines and PBMCs|
|Preparation method||MOLT-4 or U937 or PBMC cells are treated with DMF or TMF or PMF, 5-MF or 2'-MF at various concentrations for 24 h, then the cell viability is determined by the MTT assay.|
|Incubation time||24 h|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||10 mM in DMSO|
Identification of 5-Methoxyflavone as a Novel DNA Polymerase-Beta Inhibitor and Neuroprotective Agent against Beta-Amyloid Toxicity.
Merlo S, et al. J Nat Prod. 2015 Nov 25;78(11):2704-11. PMID: 26517378.
Methoxyflavone derivatives modulate the effect of TRAIL-induced apoptosis in human leukemic cell lines.
Wudtiwai B, et al. J Hematol Oncol. 2011 Dec 21;4:52. PMID: 22185222.
|Related DNA/RNA Synthesis Products|
Brivudine is a uridine derivative and nucleoside analog with pro-apoptotic and chemosensitizing properties. It is incorporated into the viral DNA and blocks the action of DNA polymerases, thus inhibiting viral replication.
Madrasin is a potent and cell penetrant splicing inhibitor that interferes with the early stages of spliceosome assembly.
Favipiravir (T-705) is a potent and selective RNA-dependent RNA polymerase inhibitor, used to treat influenza virus infections.
Vidarabine is an antiviral drug by interfering with the synthesis of viral DNA, used to treat herpes simplex and varicella zoster viruses.
Uridine is a nucleoside, contains a uracil attached to a ribose ring via a β-N1-glycosidic bond.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.