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5(6)-FITC

Cat. No. M13761
5(6)-FITC Structure
Synonym:

Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers

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100mg USD 50  USD50 In stock
500mg USD 150  USD150 In stock
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Biological Activity

5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

Chemical Information
Molecular Weight 389.38
CAS Number 27072-45-3
Solubility (25°C) DMSO ≥ 100 mg/mL
Storage 2-8°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Viki Raz Lepsky, et al. Biomolecules. FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels

[2] Yanhai Feng, et al. PLoS One. Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy

[3] Anni Woting, et al. Nutrients. Small Intestinal Permeability and Gut-Transit Time Determined with Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice

[4] Yousef Amini, et al. Biomed Mater Eng. Different methods to determine the encapsulation efficiency of protein in PLGA nanoparticles

[5] Morris J Robins, et al. J Med Chem. Improved syntheses of 5'-S-(2-aminoethyl)-6-N-(4-nitrobenzyl)-5'-thioadenosine (SAENTA), analogues, and fluorescent probe conjugates: analysis of cell-surface human equilibrative nucleoside transporter 1 (hENT1) levels for prediction of the antitumor effi䲧璵Ỵ璶㧀璴羹癦

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Keywords: 5(6)-FITC, Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers supplier, Fluorescent Dye, inhibitors, activators

 
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