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Cat. No. M4753
4-Hydroxyisoleucine Structure
Size Price Availability Quantity
20mg USD 240 In stock
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Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

4-Hydroxyisoleucine was demonstrated to stimulate glucose-dependent insulin secretion by a direct effect on pancreatic islets. In addition to stimulating insulin secretion, 4-hydroxyisoleucine reduced insulin resistance in muscle and/or liver by activating insulin receptor substrate-associated phosphoinositide 3 (PI3) kinase activity.

Customer Product Validations & Biological Datas
Source Mol Med Rep (2015). Figure 5. 4-Hydroxyisoleucine
Method western blotting
Cell Lines IR HepG2 cells
Concentrations 5, 10, 20 and 40 μmol/l
Incubation Time 24 h
Results The results shown in Fig. 5B demonstrate that 4‑HIL dose‑dependently upregulated IRS‑1 and GLUT4 expression, downregulated the expression of p‑IRS‑1 and had no effect on IRS‑2 expression in IR HepG2 cells.
Source Mol Med Rep (2015). Figure 4. 4-Hydroxyisoleucine
Method western blotting
Cell Lines IR HepG2 cells
Concentrations 5, 10, 20 and 40 μmol/l
Incubation Time 24 h
Results Notably, 4‑HIL dose‑dependently downregulated and upregulated TACE and TIMP3 expression in IR HepG2 cells, respectively
Cell Experiment
Cell lines HepG2 cells
Preparation method The established IR HepG2 cells were then treated with different concentrations of 4-HIL (0, 5, 10, 20 and 40 µmol/l) for 24 h and glucose uptake was determined as described above.
Concentrations 0, 5, 10, 20 and 40 µmol/l
Incubation time 24 h
Animal Experiment
Animal models Male Sprague-Dawley rats
Dosages 10, 30, 100 mg/kg
Administration p.o.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 147.17
Formula C6H13NO3
CAS Number 781658-23-9
Purity >98%
Storage at -20°C

Effects of 4-hydroxyisoleucine from Fenugreek Seeds on Depression-like Behavior in Socially Isolated Olfactory Bulbectomized Rats.
Kalshetti PB, et al. Pharmacogn Mag. 2015 Oct;11(Suppl 3):S388-96. PMID: 26929572.

4-Hydroxyisoleucine improves insulin resistance in HepG2 cells by decreasing TNF-α and regulating the expression of insulin signal transduction proteins.
Gao F, et al. Mol Med Rep. 2015 Nov;12(5):6555-60. PMID: 26352439.

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Keywords: 4-Hydroxyisoleucine supplier, Others, inhibitors

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