Free shipping on all orders over $ 500

3-TYP

Cat. No. M8978
3-TYP Structure
Synonym:

3-(1H-1,2,3-triazol-4-yl) pyridine

Size Price Availability Quantity
10mg USD 120 In stock
50mg USD 350 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

3-TYP is a selective SIRT3 inhibitor with an IC50 value of 16 nM, which is more potent over SIRT1 and SIRT2. 3-TYP significantly attenuates melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity in HepG2 cells exposed to Cd.

In vivo, 3-TYP significantly decreases SIRT3 activity and increases the acetylation of SOD2 compared with that in the control group, without influencing SIRT3 expression. 3-TYP attenuates the cardioprotective effects of melatonin by decreasing the LVEF and LVFS after 24 hour of reperfusion. 3-TYP also increases the infarct size, serum LDH levels, and apoptotic ratio compared with those in the IR+Mel group.

Protocol
Cell Experiment
Cell lines HepG2 cells
Preparation method Cells are randomly divided into the following treatment groups: 10 μM Cd; melatonin (1 μM)+10 μM Cd; 3-TYP (50 μM)+10 μM Cd; and 3-TYP (50 μM)+melatonin (1 μM)+10 μM Cd. cells are treated for 12 h. The role of the SIRT3-SOD2 pathway in hepatic cell protection after melatonin pretreatment is investigated.
Concentrations 50 μM
Incubation time 12 h
Animal Experiment
Animal models Sprague-Dawley rats
Formulation ethanol (70%), propylene glycol (20%), NaHCO3 (10%)
Dosages 5 mg/kg
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 146.15
Formula C7H6N4
CAS Number 120241-79-4
Purity >99%
Solubility DMSO: ≥ 25 mg/mL
Storage at -20°C
References

Melatonin ameliorates myocardial ischemia reperfusion injury through SIRT3-dependent regulation of oxidative stress and apoptosis.
Zhai M, et al. J Pineal Res. 2017 Sep;63(2). PMID: 28500761.

Polydatin ameliorates injury to the small intestine induced by hemorrhagic shock via SIRT3 activation-mediated mitochondrial protection.
Zeng Z, et al. Expert Opin Ther Targets. 2016 Jun;20(6):645-52. PMID: 27067422.

SIRT3-SOD2-mROS-dependent autophagy in cadmium-induced hepatotoxicity and salvage by melatonin.
Pi H, et al. Autophagy. 2015;11(7):1037-51. PMID: 26120888.

Related Sirtuin Products
OSS_128167

OSS_128167 is a specific SIRT6 inhibitor with IC50 values of 89, 1578 and 751 μM for SIRT6, SIRT1 and SIRT2, respectively.

Thiomyristoyl

Thiomyristoyl is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM. It inhibits SIRT1 with an IC50 value of 98 μM and does not inhibit SIRT3 even at 200 μM.

Nicotinamide

Nicotinamide is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.

Fisetin

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

Sirtinol

Sirtinol is a specific SIRT1 and SIRT2 inhibitor with IC50 of 131 μM and 38 μM, respectively.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: 3-TYP, 3-(1H-1,2,3-triazol-4-yl) pyridine supplier, Sirtuin, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.